DSPE-PEG 2000
CAS No. : 892144-24-0
(Synonyms: (C2H4O)nC42H82NO10P)
| Size | Price | Stock |
|---|---|---|
| 25mg | $80 | In-stock |
| 50mg | $125 | In-stock |
| 100mg | $200 | In-stock |
| 250mg | $350 | In-stock |
| 500mg | $550 | In-stock |
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| Cat. No. : | HY-142979 |
| M.Wt: | 1000.00 |
| Formula: | (C2H4O)nC42H82NO10P |
| Purity: | >98 % |
| Solubility: | Ethanol : 25 mg/mL (ultrasonic;warming;heat to 60°C);H2O : 25 mg/mL (ultrasonic;warming;heat to 60°C);DMSO : 50 mg/mL (ultrasonic) |
DSPE-PEG 2000 is a PEG polymer containing DSPE and amine end groups. DSPE-PEG 2000 can be used to form micelles as nanoparticles for drug delivery[1][2].
In Vitro: Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
Reparation of siRNALNPs[2]:
1.DSPE-PEG2000 (0.11 mg) in chloroform (3 mL), and transfer the lipid solution to a round bottom flask after ultrasonic treatment to fully dissolve it.
2.Steam under vacuum conditions for 30 min (75 r/min, room temperature) to allow the organic solvent to completely evaporate and obtain a homogeneous lipid film.
3.Hydrate with 3 mL of enzyme free water for 10 min (100 r/min) and then homogenize with ultrasound.
4.siRNA solution was mixed with LNPs by electrostatic interaction at a weight ratio of 1:30, and concentrated by centrifugation in a Macrosep Advance filtration device (MAP001C37) (3000g/min, 4 °C).
5.The molecular weight was cut off to 10 kDa to remove the unsealed drug.
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