AkaLumine (hydrochloride)

AkaLumine hydrochloride is a D-luciferin (HY-12591A) analogue with a K_m of 2.06 μM for recombinant firefly luciferase (Fluc) protein. AkaLumine hydrochloride emits near-infrared (NIR) light (λmax=677 nm) in reactions with native Fluc. AkaLumine hydrochloride has high tissue-penetration and increases detection sensitivity from deep-tissue targets^[1]. IC50 & Target:Km: 2.06 μM (recombinant Fluc protein)^[1]. In Vitro:1. Solution placement^[2]
1.1 Control liquid
Dissolved in: Waterless DMSO or ultra-pure water.
Level: 10 mM (as shown: 3.39 mg AkaLumine hydrochloride, 1 mL solution).
Storage: After separation, store at -20°C, protected from light, avoid cooling.
1.2 Working fluid control
Use PBS or serum-free cell culture at a concentration of 1-100 μM (based on advanced technology).
Note: For displaying working fluid, avoid light.

2. Cyst staining
2.1 For general cell use 100 μL PBS dispersed in a 96-hole plate, density 2×10⁵.
2.2 AkaLumine hydrochloride (100 µM) total 5 mM ATP magnesium (HY-B2176) in cells.
2.3 Use biological photoimaging system for continuous analysis.
In both LLC/luc and MDA-MB-231/luc cells treated with AkaLumine hydrochloride, the signal is maximal at lower concentrations (by 2.5 μM), whereas the bioluminescence generated by D-luciferin and CycLuc1 increase as concentrations increase further, not appearing to reach a maximal signal even at 250 μM^[1]. In Vivo:Subcutaneous/orthotopic tumor mouse imaging^[1]
1. Inject tumor cells into mice subcutaneously or intratibially.
2. Inject different concentrations of AkaLumine hydrochloride (0.5-33 mM; 100 μL) intraperitoneally.
3. Detect using a bioluminescence instrument 15 minutes after injection.