D-Trehalose, Carrier Protein

D-Trehalose is a disaccharide formed by a 1,1-glycosidic bond between two α-glucose units and is widely used as a food ingredient and pharmaceutical excipient. D-Trehalose is known to protect and stabilise proteins. For long-term storage, recombinant protein solution should be diluted further with 5% D-Trehalose, Carrier Protein. In Vitro:D-Trehalose is a typical stress metabolite that forms a unique protective film on cell surfaces under harsh environmental conditions such as high temperature, extreme cold, high osmotic pressure, and desiccation, thereby maintaining the life processes and biological characteristics of living organisms.
1. Stabilizes cell membranes and protein structures
2. Resists desiccation
3. Pharmaceutical excipient, used as an excipient
4. Inhibits collagen degradation
Plastic tube walls strongly adsorb proteins, causing recombinant proteins to adhere to the tube walls and be difficult to separate. This results in a low actual concentration in solution, ultimately manifesting as decreased activity. When reconstituting recombinant proteins, the addition of carrier proteins can pre-block the protein binding sites on the plastic tube walls, preventing the recombinant protein from adhering to the tube walls and thus protecting protein activity. When performing serum-free culture, in vivo experiments, or protein labeling, the recombinant protein used should not contain animal or human proteins such as BSA, FBS, or HSA. Trehalose can be used as a carrier protein for long-term storage of recombinant proteins. To maintain a certain pH and salt concentration and prevent protein instability, dissolving the carrier protein in water is not recommended. Instead, a buffer with a near-neutral pH and sufficient buffering capacity, such as PBS or culture medium (RPMI1640, DMEM), is recommended.