5,6,7-Trimethoxyflavone

5,6,7-Trimethoxyflavone is a flavonoid compound that can be isolated from plants Callicarpa japonica. 5,6,7-Trimethoxyflavone exhibits antiviral and anti-inflammatory activities through inhibition of NF-κB/AP-1/STAT signaling pathway^[1][2]. IC50 & Target:p38-α MAPK^[1] In Vitro:5,6,7-Trimethoxyflavone exhibits inhibitory effects on herpes simplex virus type 1 (HSV-1), human cytomegalovirus (hCMV), and poliovirus, inhibits strains HSV-1 HF, HSV-1 KOS, HSV-1 A4-3, HCMV Towne and Poliovirus P1 Sabin with IC₅₀s of 3.2 mg/L, 5.8 mg/L, 9.4 mg/L, 8 mg/L and 32 mg/L, respectively^[1].
5,6,7-Trimethoxyflavone exhibits slight cytotoxicity, CC₅₀s for Vero and MRC-5 are 263.13 mg/L and 37.42 mg/L^[1].
5,6,7-Trimethoxyflavone (10-40 µM, 24 h) inhibits the production of NO, PGE2, iNOS and COX-2, reduces the mRNA expression of TNF-α, IL-1β and IL-6 in LPS (HY-D1056)-stimulated RAW 264.7 cells^[2].
5,6,7-Trimethoxyflavone inhibits the transcriptional activity and nuclear translocation of NF-κB, AP-1 and STAT1/3, inhibits the phosphorylation and degradation of IκBα, and inhibits the phosphorylation of p38 MAPK^[2]. In Vivo:5,6,7-Trimethoxyflavone (10-40 mg/kg, ip, single dose) exhibits anti-inflammatory activity, prolongs the survival time of LPS (HY-D1056)-induced sepsis mice^[2].