2-Hexyl-4-pentynoic acid

2-Hexyl-4-pentynoic acid ((±)-2-Hexyl-4-pentynoic acid), a Valproic acid (HY-10585) derivative, exhibits potential roles of HDAC inhibition (IC₅₀ = 13 μM) and HSP70 induction. 2-Hexyl-4-pentynoic acid causes histone hyperacetylation and protect against glutamate-induced excitotoxicity in cultured neurons. 2-Hexyl-4-pentynoic acid can be used for the study of breast carcinoma. 2-Hexyl-4-pentynoic acid is a click chemistry reagent, it contains an Alkyne group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing Azide groups^[1][2]. In Vitro:2-Hexyl-4-pentynoic acid (10-100 μM; 7 days) blocks glutamate-induced cell death in cerebellar granule cells^[1].
2-Hexyl-4-pentynoic acid (5 μM, 10 μM, 25 μM, 50 μM, 100 μM) increases acetylated histone H3 (Ac-H3) levels in cerebellar granule cells^[1].
2-Hexyl-4-pentynoic acid (50 μM) increases HSP70-1a and HSP70-1b mRNA levels^[1].
2-Hexyl-4-pentynoic acid (HPTA; 15 μM) inhibits breast cancer cell growth and sensitizing the tumor cells to Hydroxyurea (HY-B0313) on MCF7 cells, EUFA423 cells, MCF7 cells with defective RPA2-p gene and primary culture cells derived from tissue-transformed breast tumor cells. 2-Hexyl-4-pentynoic acid induces DNA double-strand breaks, the homologous recombination is inhibited through the interference of the hyperphosphorylation of replication protein A2 and recombinase Rad51^[2].