Licoflavone B

Licoflavone B is an orally active flavonoid, with IC₅₀ values of 23.78 μM and 31.5 μM against SmATPase and SmADPase of Schistosoma mansoni, respectively. Licoflavone B selectively targets viral RdRp, inhibits viral replication, and reduces the expression levels of viral NP and PB2. Licoflavone B inhibits c-Myc expression and suppresses cancer cell proliferation. Licoflavone B disrupts the tegument of worms, reduces egg production, and induces the death of adult Schistosoma mansoni. Licoflavone B blocks the activation of the MAPK pathway, maintains colonic barrier integrity, inhibits colonic cell apoptosis, and reshapes the gut microbiota. Licoflavone B can be used in research related to influenza, multiple myeloma, schistosomiasis, and ulcerative colitis^[1][2][3][4]. IC50 & Target:IC50: 23.78 µM (S. mansoni ATPase), 31.50 µM (S. mansoni ADPase)^[1] In Vitro:Licoflavone B (0-200 μM; 24 h) potently inhibits the replication of influenza A virus A/Puerto Rico/8/1934 (H1N1) in MDCK-Gluc cells, with IC₅₀ values of 6.7 μM (0 h) and 24.7 μM (6 h), respectively^[1].
Licoflavone B (0-200 μM; 18 h) selectively inhibits the RdRp activity of influenza A virus A/Puerto Rico/8/1934 (H1N1) in MDCK-Gluc cells, with an IC₅₀ value of 9.9 μM^[1].
Licoflavone B (3.125-200 μM; 24-72 h) dose-dependently reduces viral RNA loads in MDCK-Gluc cells infected with influenza A/Puerto Rico/8/1934 (H1N1), A/Darwin/9/2021 (H3N2) and B/Beijing/ZYY-B18/2018 viruses^[1].
Licoflavone B (0-50 μM; 24 h) reduces the expression of viral NP and PB2 proteins in MDCK-Gluc cells infected with influenza A virus A/Puerto Rico/8/1934 (H1N1) in a dose-dependent manner^[1].
Licoflavone B (0-200 μM; 24 h) exhibits low cytotoxicity in MDCK-Gluc cells^[1].
Licoflavone B (1-20 μM; 48 h) inhibits the proliferation of human multiple myeloma cell line RPMI-8226 in a dose-dependent manner^[2].
Licoflavone B (10 μM; 48 h) significantly downregulates the mRNA and protein expression levels of c-Myc in human multiple myeloma cell line RPMI-8226^[2].
Incubation with Licoflavone B (5-100 μM; 24-48 h) for 24 h in vitro causes 100% mortality, complete loss of motility, and extensive tegumental damage in adult *Schistosoma mansoni* at concentrations of 25, 50, and 100 μM, while no activity is observed at 5 and 10 μM^[3].
Licoflavone B (2.5-10 μM; up to 120 h) inhibits egg-laying behavior of adult *Schistosoma mansoni* pairs in a dose-dependent manner, and complete inhibition is achieved at the concentration of 10 μM following 5 days of in vitro incubation^[3].
Licoflavone B (10-50 μM) causes dose-dependent tegumental damage to adult male *Schistosoma mansoni* in vitro and reduces the number of intact tubercles; at a concentration of 50 μM, the intact tubercles of adult worms disappear completely^[3].
Licoflavone B (5-40 μM; 30 min pre-incubation) potently inhibits the activities of ATPase and ADPase in adult *Schistosoma mansoni* homogenates in vitro, with IC₅₀ values of 23.78 μM and 31.50 μM, respectively^[3]. In Vivo:Licoflavone B (40-120 mg/kg; p.o., daily; 14 days) dose-dependently ameliorates DSS-induced ulcerative colitis in male C57BL/6 mice, with the 120 mg/kg dose exerting significant efficacy via reducing body weight loss, DAI score, histological damage, colonic inflammation, epithelial apoptosis, and MAPK pathway activation, while preserving gut barrier integrity and reshaping intestinal microflora^[4].