Hoechst 34580 (trihydrochloride)

Hoechst 34580 trihydrochloride is a cell-permeable fluorescent dye for staining DNA and nuclei. Storage: protect from light. IC50 & Target:Amyloid-β^[1] In Vitro: Hoechst 34580 prefers to locate near hydrophobic regions with binding affinity with the inhibition constant IC₅₀ of 0.86±0.05 μM for Hoechst 34580. Hoechst 34580 is a good candidate for treating the Alzheimer’s disease by inhibiting Aβ formation. 50 μM Aβ42 solutions co-incubated with 100, 25, 12.5, 3.125, 0.78, and 0.1, 0.01 μM Hoechst 34580 at 37 °C for 70 h. Hoechst 34580 can inhibit the aggregation of Aβ42 in a dose-dependent manner. And the half-maximal concentration (IC₅₀) is examined. The IC₅₀ is obtained by measuring the concentration of Hoechst 34580 while maintaining the Aβ42 concentration which gave 0.86±0.05 μM for Hoechst 34580^[1].