Ribonuclease T1, Aspergillus oryzae

Ribonulease T1, Aspergillus oryzae (Rnase T1), is commonly used in biochemical research. Ribonuclease T1 is an endonuclease that can specifically degrade single stranded RNA. Ribonuclease T1 can form nucleoside 2 ', 3 '-cyclic phosphoric acid intermediates to cut the phosphodiester bond between 3' -guanosine residues and adjacent nucleoside 5 '-OH groups to produce 3' -GMP terminal oligonucleotides^[1]. In Vitro: Product Information
Molecular weight: 11.2 kDa monomer
Inhibitors: metal ions Mg²⁺, Ca²⁺, Zn²⁺, Fe²⁺, Cu²⁺ (100 mM MgCl₂ has an inhibition rate of about 40%, 10 mM CaCl₂ has an inhibition rate of about 30%); mononucleotides (2’-GMP, 3’-GMP, etc.); guanylate-2’,5’-guanosine is a specific inhibitor
Storage solution: 50 mM Tris-HCl (pH 7.4), 50% (v/v) glycerol

Digestion of RNA
It is recommended to add 0.2 μL RNase T1 per μg RNA, dissolved in 50 mM Tris-HCl (pH 7.5) and 1 mM EDTA. Incubate at 37°C for 30 min.