L-Asparaginase

L-Asparaginase (L-ASNase) is a deamidating enzyme that catalyses the hydrolysis of L-asparagine and L-glutamine, and can be used for the research of acute lymphoblastic leukemia. L-Asparaginase depletes L-asparagine from plasma resulting in inhibition of RNA and DNA synthesis with the subsequent blastic cell apoptosis^[1]. In Vitro:L-Asparaginase has a cytotoxic effect on AML blasts and LSC subpopulations^[1].
L-Asparaginase is effective on both CD34⁺CD38⁺ and CD34⁺CD38⁺ LSC fractions, MSC and monocytes/macrophages partially counteracted the effect^[1].
Although lyophilized L-asparaginase is stable at room temperature for 3 weeks, it should be stored dry below -18°C. L-Asparaginase should Co-culture with cells at 37°C, 5% CO2 for 24, 48 h.
L-Asparaginase (100 IU/mL, 0-24 h) is co-cultured with L5178Y and B16 cells (5×10⁵/mL), and cells are arrested in the G1 phase and DNA strand breaks occurred^[2].
Product Information
Optimal pH: 8.0-9.0
Agonists: Anaerobic conditions, amino acids
Inhibitors: 5-diazo-4-o-L-norvaline (DONV), dimethyl sulfoxide, glutaraldehyde, L-asparagine and L-aspartic acid, NH₄⁺, trypsin

Instructions
Dissolve in 1 mg/ml 0.2 M Tris-HCl pH 8.6, store at 2-8℃ after dissolution, and use as soon as possible within 1 week. Or dissolve according to specific experimental references.