Pepsin (MS Grade)

Pepsin (MS Grade) is the major pig and human gastric proteases, it is a pepsin-like minor gastric proteolytic enzymes. Pepsin (MS Grade) contributes to proteolysis of food proteins in the vertebrate stomach^[1]. In Vitro:This product can be used for: 1) protein analysis in mass spectrometry and other applications; 2) for structural protein research (HDX exchange) and antibody analysis; 3) for digestion of tightly folded proteins in proteomics; 4) digestion of casein for vaccine production protein.
Protocol
1) Dissolve the target protein: Dissolve the protein in pH 1.0-5.0 buffer;
2) Enzyme dissolution: Dissolve pepsin powder in double-distilled water. Note that the pH should be lower than 5.5 so that the final concentration of pepsin is 1 mg/mL;
3) Enzyme digestion: Add pepsin to the target protein solution. The recommended ratio is enzyme: protein = 1:20-1:100 (W: W). After mixing, react at 37°C for 1-18 hours and heat at 95°C. Stop the reaction after 10 minutes.