Papain (lyophilized powder, ≥10 units/mg protein)

Papain (lyophilized powder, ≥10 units/mg protein) is a cysteine protease of the peptidase C1 family. Papain (lyophilized powder, ≥10 units/mg protein) enhances red cell agglutination by anti-D and anti-A, and increases red cell sensitivity to K cell-mediated lysis in ADCC assays. Papain (lyophilized powder, ≥10 units/mg protein) can induce pulmonary emphysema. Papain (lyophilized powder, ≥10 units/mg protein) can be used for the researches of Rh haemolytic disease of the newborn and pulmonary emphysema^[1][2]. In Vitro:This product requires activation before use. Activation method:
Incubate the enzyme in a solution containing 1.1 mM EDTA, 0.067 mM mercaptoethanol, and 5.5 mM cysteine-HCl for 30 minutes.

Papain (lyophilized powder, ≥10 units/mg protein) (5 U/mL; 15 min) pretreatment of O Rh-positive red cells enhances anti-D-mediated agglutination, while it slightly reduces anti-A-mediated agglutination of A Rh-negative red cells^[2].
Papain (lyophilized powder, ≥10 units/mg protein) (5 U/mL; 15 min) pretreatment of O Rh-positive red cells increases their sensitivity to lymphocyte-mediated lysis in anti-D-mediated ADCC assays at low antibody concentrations and a 15:1 effector-to-target ratio^[2].
Papain (lyophilized powder, ≥10 units/mg protein) (5 U/mL; 15 min) pretreatment of O Rh-positive target red cells enables significant PBMC-mediated lysis in anti-D-mediated ADCC assays at a 1:3:3 effector-to-target-to-competitor ratio^[2].
Papain (lyophilized powder, ≥10 units/mg protein) (5 U/mL; 15 min) pretreatment of A Rh-negative target red cells enables significant PBMC-mediated lysis in anti-A-mediated ADCC assays at a 1:3:3 effector-to-target-to-competitor ratio^[2]. In Vivo:Papain (lyophilized powder, ≥10 units/mg protein) (10 IU; intratracheal instillatio; at day 0 and 7) induces pulmonary emphysema in female BALB/c mice^[1].