Diphenylcyclopropenone

Diphenylcyclopropenone (Diphencyprone) is a potent hapten acting as a topical immunomodulatory agent, which induces an allergic contact dermatitis. Diphenylcyclopropenone induces an increase of cell-surface thiols in cells of a human monocytic cell line, THP-1. Diphenylcyclopropenone acts on the autoreactive T-lymphocytes within the follicular milieu to induce Apoptosis. Diphenylcyclopropenone can be used for alopecia areata research^[1][2][3][4]. In Vitro:Diphenylcyclopropenone (4-38 μM, 2 h) induces an increase of cell-surface thiols in THP-1 cells and primary monocytes, leading to activation of signal transduction in the MAPK pathway via IRE1α, resulting in ER stress^[3].
In Vivo:Diphenylcyclopropenone (1.0 or 0.1 % (w/v), exposed on the dorsal sides of both ears, once daily for 3 days followed by challenge twice per week for 8 weeks) increases average ear thickness, modulates B-cell and T-cell activations, increases the proportion of IFN-γ-positive CD8⁺ cells in Pancreatic lymph nodes in mice^[4].