BCECF
CAS No. : 85138-49-4
(Synonyms: 2′,7′-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein)
| Size | Price | Stock |
|---|---|---|
| 1mg | $65 | In-stock |
| 5mg | $300 | In-stock |
| 10mg | $420 | In-stock |
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| 100 mg | Get quote | |
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| Cat. No. : | HY-101882 |
| M.Wt: | 520.44 |
| Formula: | C27H20O11 |
| Purity: | >98 % |
| Solubility: | DMSO : 62.5 mg/mL (ultrasonic) |
BCECF is a pH-sensitive fluorescent dye. BCECF allows measurements in the physiological pH range 6.0–8.0. Excitation ratio: 490/440 nm; Emission intensity: 535 nm.
In Vitro:Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
1. Prepare a 2-20 mM stock solution of BCECF in DMSO and store at -20℃.
2. Prepare a 5-50 μM BCECF dye-loading solution in buffer solutions (Hepes, pH 7.4 or PBS).
3. Add 1000 μL/well (6-well plate),100 μL/well (96-well plate) or 25 μL/well (384-well plate) BCECF dye-loading solution into the cell plate.
4. Incubate the dye-loading plate in a cell incubator about 30minutes.
5. Run the pH assay by monitoring the fluorescence at Ex/Em = 490/535 nm or 430/535 nm for ratio measurements.
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