Ampyrone

Ampyrone (4-Aminophenazone; 4-Aminoantipyrine) is a reversible and low-damage optical clearing agent and non-selective COX inhibitor based on UV absorption properties. Ampyrone can improve the optical transmittance of mouse skin and other tissues. Ampyrone can induce tissue refractive index matching by enhancing UV absorption, reduce light scattering, and achieve tissue transparency in vivo. Ampyrone reduces the synthesis of prostaglandin PGE2, thereby exerting anti-inflammatory, analgesic and antipyretic effects. Ampyrone inhibits DNA damage, cell apoptosis and immune cell phagocytosis induced by Doxorubicin (HY-15142A) and Cisplatin (HY-17394), etc., and participates in the regulation of toxicity in tumor chemotherapy^[1][2][3]. In Vivo:Ampyrone (12-24 mg/kg; intraperitoneal injection; single dose; 72 h) is non-genotoxic and non-mutagenic in male adult mice. However, when Ampyrone is used in combination with Doxorubicin (HY-15142A), Cisplatin (HY-17394) or Cyclophosphamide (HY-17420), Ampyrone reduces the induced DNA damage, micronucleus formation, cell apoptosis and spleen phagocytic activity^[1].
Ampyrone supports structural (such as YFP-labeled neurons) and functional (such as GCaMP calcium signals) imaging of deep tissues (such as the cerebral cortex), and is suitable for longitudinal dynamic observations in living animals, such as long-term tracking of neurons in the awake mouse brain and real-time monitoring of neural activity^[2].