Fluorol Yellow 088

Fluorol Yellow 088 is a fluorescent dye that can be used as a biological material or an organic compound for life science research. Fluorol Yellow 088 is suitable for lipid staining in plant tissues, particularly for the fluorescence imaging of suberized cell walls and hydrophobic structures, with an excitation wavelength of 365 nm and an emission wavelength of ≥420 nm^[1][2]. In Vitro:Lipid Staining in Plant Tissue^[1]
Fluorol Yellow 088 is a highly efficient fluorescent lipid dye suitable for lipid staining in plant tissues, particularly for the fluorescence imaging of suberized cell walls and hydrophobic structures.
I. Reagent Preparation
Staining solution:
(1) Prepare a 0.01% (w/v) solution of Fluorol Yellow 088.
(2) Dissolve the dye in polyethylene glycol (PEG 400) and heat at 90°C for 1 hour to ensure complete dissolution.
(3) Add an equal volume of 90% glycerol solution (containing 10% distilled water) and mix thoroughly.
II. Staining Procedure
Sample fixation:
(1) Use fresh tissue or 50% ethanol-fixed tissue for staining.
(2) Prepare thin sections using a razor blade or a microtome to ensure section integrity.
Sample staining:
(1) Immerse tissue sections in Fluorol Yellow 088 staining solution and incubate at room temperature for 1 hour.
(2) Lightly rinse the tissue to remove excess dye (brief rinsing is recommended to avoid excessive removal of the stain).
Mounting:
Use 75% glycerol as the mounting medium (to prevent fading).
III. Observation and Analysis
Fluorescence microscopy observation: excitation wavelength 365 nm, emission wavelength ≥ 420 nm.
Imaging can be recorded using Nomarski interference-contrast or fluorescence microscopy.
IV. Application Scope
Primarily used for lipid staining in plant tissues, especially for the visualization of suberin lamellae (Suberin lamellae).
Effectively stains endodermis (Endodermis) and exodermis (Exodermis).
Advantages:
Strong staining stability, not prone to precipitation in aqueous media.
High lipid specificity, providing high-contrast imaging.
Suitable for large-scale scanning (rapid screening under low magnification).