3-Carboxy-7-Hydroxycoumarin

3-Carboxy-7-Hydroxycoumarin is a 7-hydroxycoumarin (umbelliferone) fluorescent dye and antibody-conjugated labeling agent with UV excitation properties. 3-Carboxy-7-Hydroxycoumarin undergoes deprotonation in neutral-to-alkaline environments, producing strong blue fluorescence. The absorption/emission maxima of 3-Carboxy-7-Hydroxycoumarin are 386/448 nm. 3-Carboxy-7-Hydroxycoumarin is applicable for related research such as fluorescent probe synthesis and covalent protein labeling^[1][2]. In Vitro:3-Carboxy-7-Hydroxycoumarin can be chemically activated to generate NHS ester derivatives for covalent bioconjugation with amino-containing biomacromolecules^[1].

3-Carboxy-7-Hydroxycoumarin (compound 2) exhibits a pK_a of 7.5, absorption/emission maxima at 386/448 nm, an extinction coefficient of 36.7 × 10³, and a quantum yield of 0.70 in 0.1 M phosphate buffer at pH 10^[1][2]. In Vivo:3‑Carboxy‑7‑Hydroxycoumarin‑derived chlorinated analogue V450 is suitable for multicolor immunophenotyping panels and yields bright, well‑separated cell populations^[1].