2',7'-Dichlorofluorescein

2',7'-Dichlorofluorescein is an important fluorescent product in the detection of oxidative stress and can be used to measure reactive oxygen species (ROS) (Ex=503 nm and Em=523 nm)^[1][2]. In Vitro:The precursor of 2',7'-Dichlorofluorescein, 2',7'-Dichlorodihydrofluorescein diacetate, is lipophilic and can freely cross the cell membrane. After entering the cell, under the action of intracellular esterases, 2',7'-Dichlorodihydrofluorescein diacetate is converted into 2′,7′-Dichlorodihydrofluorescein (HY-153006). 2′,7′-Dichlorodihydrofluorescein is somewhat hydrophilic and remains inside the cell. When oxidants are present in the cell, it is oxidized to generate 2',7'-Dichlorofluorescein^[1].