Rhodamine B hydrazide
CAS No. : 74317-53-6
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|---|---|---|
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| Cat. No. : | HY-123645 |
| M.Wt: | 456.58 |
| Formula: | C28H32N4O2 |
| Purity: | >98 % |
| Solubility: | DMSO : 100 mg/mL (ultrasonic) |
Rhodamine B hydrazide is a fluorescent derivative based on rhodamine B, containing the spirocyclic structure of Rhodamine B (HY-Y0016), which can be used to detect copper ions (Cu2+), mercury ions, peroxynitrite, hydroxyl radicals and nitric oxide (NO)[1][2][3].
Excitation/emission wavelength:
Conventional detection: 510/578 nm.
Sulfite detection: 554 nm absorption, 574 nm emission (due to the formation of Rhodamine B fluorescent product).
In Vitro:Rhodamine B hydrazide is a colorless and non-fluorescent rhodamine derivative. While the emission is related to the concentration of sulfite (5-800 ng/mL; detection limit=1.4 ng/mL (3σ)). Sulfite reduces dissolved oxygen to yield superoxide radicals, which binds to Rhodamine B hydrazide to form Rhodamine B. Rhodamine B hydrazide gives Rhodamine B-like fluorescence in the presence of sulfite, which is enhanced by Tween 80 surfactant micelles. Rhodamine B hydrazide has an absorption maximum at 554 nm and a fluorescence emission maximum at 574 nm[1].
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