TES

TES is an excellent hydrogen ion buffer. TES has characteristics such as an appropriate dissociation constant, a low metal-binding constant, and high water solubility. TES can be used in the research of mammalian cell culture, viruses, and so on^[1][2]. In Vitro:TES (0.1 M) has pK_a values of 7.92, 7.50, and 7.14 at 0°, 20° and 37°, respectively, and the concentration of its saturated aqueous solution at 0° is 2.6 M^[1].
TES (6-50 mM; 11-14 days) in a closed culture environment is similar to or even more effective than the NaHCO₃-CO₂-buffered medium in terms of colony-forming efficiency, colony size, and cell morphology of several mammalian cell lines (HeLa S3, KB, BHK 21-Cl3, 3Y1-B Cl-1)^[2].
TES (25-50 mM; 14-19 days) has a similar efficiency to NaHCO₃-CO₂-buffered medium and the required time is shorter in the SV40 virus plaque assay with monkey kidney CV-1 cells^[2].