Pentafluorobenzenesulfonyl fluorescein

Pentafluorobenzenesulfonyl fluorescein is a H₂O₂-selective sensor that can be used to detect H₂O₂ levels in cells. Pentafluorobenzenesulfonyl fluorescein is generally non-fluorescent, but emits fluorescence when its sulfonyl bond undergoes perhydrolysis by H₂O₂. Pentafluorobenzenesulfonyl fluorescein undergoes slight cleavage of its sulfonate ester bond by [Cu (phen)₂]²⁺, and can detect hydrogen peroxide around the ablation sites of fin tissues and keratinocytes in zebrafish larvae^[1][2][3]. In Vivo:Pentafluorobenzenesulfonyl fluorescein (4µM; incubated in media; single 1-2 hour exposure prior to imaging) detects significantly increased hydrogen peroxide levels in the caudal fin of glucose-treated larval Danio rerio, with elevated regional fluorescence at 5dpf and widespread, intensified fluorescence at 8dpf^[1].