CAS No. : 72088-94-9
(Synonyms: 5(6)-FAM; 5-(and-6)-Carboxyfluorescein mixed isomers)
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| Cat. No. : | HY-15940 |
| M.Wt: | 752.63 |
| Formula: | C42H24O14 |
| Purity: | >98 % |
| Solubility: | DMSO : 62.5 mg/mL (ultrasonic;warming;heat to 60°C) |
5(6)-Carboxyfluorescein (5(6)-FAM) is an amine-reactive pH-sensitive green fluorescent probe. 5(6)-Carboxyfluorescein (5(6)-FAM) can be used to label proteins, peptides and nucleotides. 5(6)-Carboxyfluorescein can be used for the detection of tumour areas in vivo[1][2].
In Vitro: 5(6)-Carboxyfluorescein has two main characteristics: it has two wavelengths of maximum absorbance (465 and 490 nm) and its fluorescence emission (maximum, 515 nm) increases as a function of pH in the physiological pH range of 6-7.4[2].
In Vivo: 5(6)-Carboxyfluorescein (5 mg/kg; i.p.) can be used for in vivo pH mapping of tumor tissue[2].
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