DiBAC4(3)

DiBAC4(3) is a voltage-sensitive fluorescent dye (λ_ex=490 nm, λ_em=505 nm). DiBAC4(3) can be used to measure cell membrane potential. In Vitro:1. Solution preparation
1.1 Preparation of stock solution
Solvent: DMSO
Concentration: 10-40 mM.
Storage: Store at -20°C or -80°C in dark after aliquoting. Avoid repeated freezing and thawing.
1.2 Preparation of working solution
Dilute to 10-40 µM with Hanks and 20 mM Hepes buffer (HHBS) or other buffer, such as pH 7 with 0.04% to 0.08% Poloxamer 407 (F127) (HY-D1005) and 2 mM Trypan Red (HY-D0983)).
Note: The working solution should be prepared and used immediately. Keep it away from light.

2. Cell staining
2.1 Cells are plated in clear-bottomed black 96-well microplates.
2.2 Add 100 µL DiBAC4(3) into the cell plate.
2.3 Incubate the dye-loading plate in a cell incubator for 30 to 60 minutes.
2.4 Run the membrane potential assay by monitoring the fluorescence intensity at Ex/Em = 490/505 nm.