| Size | Price | Stock |
|---|---|---|
| 25g | $19 | In-stock |
| 100g | $34 | In-stock |
| 500g | $110 | In-stock |
| 1000g | $218 | In-stock |
| > 2 kg | Get quote | |
| We match the lowest price on market. | ||
We offer a substantial discount on larger orders, please inquire via [email protected]
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Inquiry for price and availability only. Please place your order via our email or fax.
| Cat. No. : | HY-D0856 |
| M.Wt: | 209.24 |
| Formula: | C8H19NO5 |
| Purity: | >98 % |
| Solubility: | H2O : 200 mg/mL (ultrasonic) |
Bis-Tris is an amine buffer suitable for protein and nucleic acid systems with a pH buffer range of 5.8-7.2. Bis-Tris can also be mixed with HEPES and cacodylic acid buffer to create a pH 8 environment and monitor fluorescence emission intensity at 305 nm. Gels formulated with Bis-Tris also avoid protein degradation in samples prepared at higher pH (pH 8.5)[1][2][3].
In Vitro:Bis-Tris (10 mM or 4-12% Bis-Tris gel) has excellent buffering capacity and, in synergy with HEPES and cacodylic acid, covers a wide pH range. It can be used for fluorescence spectroscopy analysis, circular dichroism (CD) analysis, GST pull-down binding assay, gel filtration analysis, nuclear magnetic resonance (NMR) analysis, and protein electrophoresis. Abnormal pH fluctuations can affect experimental results[2][3].
Buffers containing Bis-Tris can be used to preserve purified proteins to prevent degradation (4°C)[2].
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