Bis-Tris


CAS No. : 6976-37-0

6976-37-0
Price and Availability of CAS No. : 6976-37-0
Size Price Stock
25g $19 In-stock
100g $34 In-stock
500g $110 In-stock
1000g $218 In-stock
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Cat. No. : HY-D0856
M.Wt: 209.24
Formula: C8H19NO5
Purity: >98 %
Solubility: H2O : 200 mg/mL (ultrasonic)
Introduction of 6976-37-0 :

Bis-Tris is an amine buffer suitable for protein and nucleic acid systems with a pH buffer range of 5.8-7.2. Bis-Tris can also be mixed with HEPES and cacodylic acid buffer to create a pH 8 environment and monitor fluorescence emission intensity at 305 nm. Gels formulated with Bis-Tris also avoid protein degradation in samples prepared at higher pH (pH 8.5)[1][2][3]. In Vitro:Bis-Tris (10 mM or 4-12% Bis-Tris gel) has excellent buffering capacity and, in synergy with HEPES and cacodylic acid, covers a wide pH range. It can be used for fluorescence spectroscopy analysis, circular dichroism (CD) analysis, GST pull-down binding assay, gel filtration analysis, nuclear magnetic resonance (NMR) analysis, and protein electrophoresis. Abnormal pH fluctuations can affect experimental results[2][3].
Buffers containing Bis-Tris can be used to preserve purified proteins to prevent degradation (4°C)[2].

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