Cestrin

Cestrin is a plant cellulose synthase complex (CESA) trafficking inhibitor. Cestrin selectively blocks the sustained trafficking of cellulose synthase complexes (CSCs) and the interaction between CSC and POM2, which indirectly causes secondary destabilization of cortical microtubules (MTs). Cestrin reduces cellulose content, inhibits anisotropic cell growth, induces cell swelling and decreases cell elongation. Cestrin is applicable to plant cell biology research^[1]. In Vitro:Cestrin (15 μM; 2 h) reduces the average velocity of GFP-CESA3 particles to 127 nm/min, causes GFP-CESA3 to disappear from the plasma membrane, and induces its accumulation in the cortical compartment of hypocotyl cells from 3-day-old etiolated Arabidopsis thaliana^[1].
Cestrin (15 μM; 2 h) does not disrupt the localization or morphology of multiple organelle and trafficking markers, indicating its selective action in hypocotyl cells of 3-day-old etiolated Arabidopsis thaliana^[1].
Cestrin (0-15 μM; 5 d) inhibits the anisotropic growth of hypocotyls in 5-day-old etiolated Arabidopsis thaliana seedlings, with an IC₅₀ value of 4.85 μM, while inducing cell swelling and reducing elongation^[1].
Cestrin (9 μM; 8 d) reduces the cellulose content of 8-day-old etiolated Arabidopsis hypocotyls by approximately 30%^[1].
Cestrin (8-15 μM; 2 h) significantly reduces the incorporation of [¹³C]glucose into cellulose and non-cellulosic cell wall components in 4-day-old etiolated Arabidopsis seedlings^[1].
Cestrin (15 μM; 2 h) reduces the average velocity of GFP-KOR1 particles to 60 nm/min in hypocotyl cells of 3-day-old etiolated Arabidopsis thaliana, causes GFP-KOR1 to disappear from the plasma membrane, and induces its accumulation in the cortical compartment^[1].
Treatment of etiolated Arabidopsis hypocotyl cells (3-day-old) with Cestrin (15 μM; 1.5-2 h) for 1.5 h reduces the average velocity of POM2/CSI1-3xYpet particles to 164 nm/min, while treatment for 2 h induces the dissociation of POM2/CSI1 from the cytoplasm^[1].
Cestrin (15 μM; 2 h) disrupts the structure and stability of cortical microtubules in hypocotyl cells of 3-day-old etiolated Arabidopsis thaliana, but does not affect the dynamics of the actin cytoskeleton^[1].
Cestrin (15 μM) does not inhibit tubulin polymerization in vitro; instead, it causes a slight but significant increase in the polymerization reaction^[1].
Cestrin (15 μM; 2 h) increases the colocalization level of GFP-CESA3 and CFP-SYP61 in hypocotyl cells of 3-day-old etiolated Arabidopsis thaliana by approximately 15%, thereby enriching CSCs in SYP61-associated compartments^[1].
Cestrin (8 μM; 5 d) inhibits hypocotyl growth of isoxaflutole-resistant mutant ixr1-1, wild-type Col-0, and prc1-1 Arabidopsis seedlings^[1].