Acridine Orange (hydrochloride)
CAS No. : 65-61-2
| Size | Price | Stock |
|---|---|---|
| 100mg | $60 | In-stock |
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| 500 mg | Get quote | |
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| Cat. No. : | HY-101879 |
| M.Wt: | 301.82 |
| Formula: | C17H20ClN3 |
| Purity: | >98 % |
| Solubility: | H2O : 50 mg/mL (ultrasonic;heat to 60°C);DMSO : 25 mg/mL (ultrasonic) |
Acridine Orange hydrochloride is a cell-penetrable nucleic acid-selective fluorescent dye. Acridine Orange hydrochloride produces orange fluorescence when it binds to ssDNA or RNA, and green fluorescence when it binds to dsDNA (Ex: 488 nM; Em: green fluorescence at 530 nm, orange fluorescence at 640 nm)[1][2][3].
In Vitro:Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs)[4].
1. Stain cells with Acridine Orange hydrochloride (1 μM; 20 min; 37℃).
2. Wash cells with PBS.
3. Cells are observed by a confocal laser scanning microscopy (FV3000, Olympus).
In Vivo:Acridine Orange (0.1 mg/kg, i.v., dogs) hydrochloride shows no clinical signs of toxicity and no abnormalities were seen in the blood within 30 days[1].
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