Dipotassium [2,2'-biquinoline]-4,4'-dicarboxylate

Dipotassium [2,2'-biquinoline]-4,4'-dicarboxylate is a chelating agent targeting Cu^I. Dipotassium [2,2'-biquinoline]-4,4'-dicarboxylate forms a water-soluble complex with Cu^I at a 2:1 stoichiometric ratio, which not only inhibits its oxidation and disproportionation reactions, but also mediates the Cu^I-catalyzed azide-alkyne cycloaddition click chemistry reaction to achieve bioconjugation of fluorophores or biotin with functionalized proteins. Dipotassium [2,2'-biquinoline]-4,4'-dicarboxylate chelates Cu⁺ from proteins that reduce Cu²⁺, forming a purple compound detectable by colorimetry, and thus is used to determine the total protein concentration in solution. Dipotassium [2,2'-biquinoline]-4,4'-dicarboxylate is a potential small-molecule candidate for AHAS inhibitors^[1][2][3][4]. In Vitro:Dipotassium [2,2'-biquinoline]-4,4'-dicarboxylate (BCA) acts as a Cu (I) chelating stabilizer in CuI-catalyzed azide-alkyne cycloaddition reactions^[1].
Dipotassium [2,2'-biquinoline]-4,4'-dicarboxylate (BCA) enables quantitative analysis of carbohydrates in three types of soil extracellular polymeric substance (EPS) extracts^[2].
Dipotassium [2,2'-biquinoline]-4,4'-dicarboxylate (Compound 8) is a potential small-molecule candidate inhibitor of AHAS identified via virtual screening. It exhibits only 1.4 % inhibitory effect on rapeseed root growth at a concentration of 100 μg/mL, and shows no inhibitory activity at 10 μg/mL^[3].
Dipotassium [2,2'-biquinoline]-4,4'-dicarboxylate (BCA) is a core reagent in commonly used BCA protein quantification methods. Under alkaline conditions, protein complexes with Cu²⁺ and is reduced to Cu⁺. Dipotassium [2,2'-biquinoline]-4,4'-dicarboxylate then binds with Cu⁺ to form a purple-blue complex. The absorbance of this complex is directly proportional to the protein concentration^[4].