Argireline

Argireline (Acetyl hexapeptide-3) is a non-toxic, skin-permeable, antiwrinkle peptide. Argireline significantly inhibits Ca²⁺ dependent neurotransmitter release (acetylcholine) at the neuromuscular junction. Argireline has antiwrinkle and anti-aging activity^[1][2][3]. In Vitro: The incubation of Argireline solution in different concentrations with human embryonic kidney HEK-293 (IC50 of 34.862 μM) and neuroblastoma IMR-32 cells (IC50 of 68.458 μM)) for 48 h have dose dependent antiproliferative effect. Argireline solution incubated in different concentrations with HSF has a dose-dependent antiproliferative effect on those cells. Cellular proliferation is not effected in Argireline low concentration^[2]. In Vivo: Argireline is applied to the aged mice twice daily for 6 weeks. There is an improvement in the histological structure of skin tissue in the aged mice; the amount of type I collagen fibers increased, while that of type III collagen fibers decreased. Argireline could improve the histological structure of skin tissue and rejuvenate the aging skin^[3].