Brilliant Blue R250
CAS No. : 6104-59-2
(Synonyms: Brilliant Blue R)
| Size | Price | Stock |
|---|---|---|
| 100g | $63 | In-stock |
| 500g | $170 | In-stock |
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| 2 kg | Get quote | |
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| Cat. No. : | HY-D0232 |
| M.Wt: | 825.97 |
| Formula: | C45H44N3NaO7S2 |
| Purity: | >98 % |
| Solubility: | DMSO : 50 mg/mL (ultrasonic);H2O : 2 mg/mL (ultrasonic;warming;heat to 60°C) |
Brilliant blue R250 (Brilliant Blue R), an anionic dye, is the most popular stain to detect proteins resolved in SDS-PAGE gels[1].
In Vitro:Stain solution preparation:
Add 100 mL of glacial acetic acid to 450 mL ultrapure water.
Dissolve the 3 g of Coomassie Dye in 450 mL methanol.
Filter the solution before use
Standard Gel Staining Protocol
1. Gel may be prefixed in 50% MeOH, 10% HoAC, and 40% H2O for 30 minutes to overnight.
2. Stain gel in the above solution, with 0.25-0.3% Coomassie Blue R-250, for 2-4 hours, until the gel is a uniform blue color. Staining is complete when the gel is no longer visible in the dye solution. Berore complete staining, the gel will appear as a lighter area against the dark staining solution.
3. Destain for 4-24 hours in 5% MeOH, 7.5% HOAC, 87.5% H2O.
4. Store gels in 7% HOAC.
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