Texas Red (solution)

Texas Red (Sulforhodamine 101) (solution) is an amphoteric rhodamine red fluorescent dye (excitation/emission: 586/605 nm). Texas Red is used extensively for investigating neuronal morphology and acts as acell type-selective fluorescent marker of astrocytes bothin vivoand in slice preparations^[1].
Solvent and concentration: DMSO: 10 mM
The 1 mL volume is defined as the base specification. All larger sizes correspond to incremental volumes of this base. In Vitro: Description: Texas Red (Sulforhodamine 101) can be used as an astrocyte marker for hippocampal slices staining with red fluorescence^[3].
Method: For cell staining.
1. Stain hippocampal slices with Texas Red (5 μM; 35 min; 34°C) in carbogenated Artificial CerebroSpinal Fluid (ACSF).
2. Return hippocampal slices to dye-free ACSF for at least 30 min before imaging.
3. Use a confocal laser scanning microscopy (Zeiss LSM 880) for image.
In Vivo: In vivo, epileptic activity can be induced by intra-hippocampal injection of small volumes of 10 μM Texas Red or topical application of 100 μM^[1].