Ethylenediaminetetraacetic acid

Ethylenediaminetetraacetic acid (EDTA) is a kind of metal chelating agent (binds to bivalent and trivalent metal cations, including calcium). Ethylenediaminetetraacetic acid has antibacterial, anti-inflammatory, antioxidant, anti-hypercalcemia and anticoagulant activities. Ethylenediaminetetraacetic acid decreases the metal ion-catalyzed oxidative damage to proteins, and allows maintenance of reducing environment during protein purification. Ethylenediaminetetraacetic acid can alleviate the liver fibrosis. Ethylenediaminetetraacetic acid can be used for coronary artery disease and neural system disease research^{[1][2][3][4][5][6][7]}. In Vitro: Ethylenediaminetetraacetic acid has a strong bactericidal effect on the cell wall of P. aeruginosa and a. faecalis^[4].
Ethylenediaminetetraacetic acid (0.005-0.01 M) has good heavy metal extraction in contaminated silty-clay-loam soil columns, can extract Pb, Cd and Zn in a concentration-dependent way with an extraction efficiency sequence of Pb > Cd > Zn^[6].
Ethylenediaminetetraacetic acid (1.2 mM) enhances the activity of the CRE driving promoter by activating T-cell death-associated gene 8 (TDAG8) in HEK293T cells, thereby enhancing the production of cAMP in the cells^[7].
When Ethylenediaminetetraacetic acid (EDTA) is prepared, NaOH is needed to adjust it in order to dissolve it. A relatively large amount of NaOH is required, and the dissolution is instantaneous, so NaOH needs to be added slowly, little by little. In Vivo: Ethylenediaminetetraacetic acid (60 mg/kg; Intraperitoneal injection; Three times per week for three weeks) can reduce liver fibrosis, lipid peroxidation and liver inflammation in CCl₄ induced liver fibrosis rats, and has antioxidant and anti-inflammatory activities^[5].