Ethacrynic acid

Ethacrynic acid has anti-inflammatory and anticancer activity. Ethacrynic acid is an orally active diuretic. Ethacrynic acid is an inhibitor of glutathione S-transferase (GSTs) and Wnt signaling pathways. Ethacrynic acid is a radiosensitizer. Ethacrynic acid can inhibit airway smooth muscle (ASM) contraction in mice. Ethacrynic acid can increase the outflow of aqueous humor from the eye for the study of glaucoma^{[1][2][3][4][5][6][7][8][9]}. In Vitro:Ethacrynic acid (50 μM; 24 h) inhibits Wnt/β-catenin signaling in CLL cells^[1].
Ethacrynic acid (1-100 μM; 48 h) is cytotoxic in CLL cells with an IC₅₀ of 8.56 μM^[1].
Ethacrynic acid (0.01-0.25 mmol/L; 30 min) increases aqueous humor outflow facility acutely in eye and the outflow rate increases from 28% to 105%^[2].
Ethacrynic acid (10-100 μM; 30 min) can inhibit the activation of NF-κB pathway in RAW264.7 cells induced by LPS (100 ng/mL) and has anti-inflammatory activity^[3].
Ethacrynic acid (20 μM/mL; 2 h) can increase the radiation intensity in MCF-7 cancer cells after radiation exposure^[3].
Ethacrynic acid (100 μmol/L; 62.5-250 min) inhibits tracheal ring contraction induced by high -K⁺ (80 mmol/L) and acetylcholine (ACh, 100 µmol/L) in a dose-dependent manner with EC₅₀ of 40.28 µmol/L and 56.22 µmol/L, respectively^[8].
Ethacrynic acid (100 μmol/L; 500-2500 s) decreases the intracellular Ca²⁺ concentration induced by high -K⁺ and ACh from 0.40 to 0.16, 0.50 to 0.39, respectively^[8].
In Vivo:Ethacrynic acid (450 μg/mouse; Oral gavage; Once daily for 60 days) can inhibit tumor growth in the mice^[5].