Tolclofos-methyl

Tolclofos-methyl is an organophosphorus fungicide. Tolclofos-methyl undergoes photoinduced oxidative degradation to form oxo-analog derivatives. Tolclofos-methyl inhibits spore division in fungi and oomycetes, induces cytoplasmic leakage, and interferes with flagellar movement. Tolclofos-methyl is rapidly metabolized and excreted in rodents. Tolclofos-methyl can be used in studies related to fungal infections^[1][2][3]. In Vitro:Tolclofos-methyl undergoes rapid photoinduced oxidation on kaolinite clay mineral films; biphasic photoinduced oxidation occurs on montmorillonite mineral films; this photoinduced oxidation is delayed by dehydration of the kaolinite and montmorillonite clay mineral films and the removal of molecular oxygen^[1].
Tolclofos-methyl completely inhibits mycelial growth of Rhizoctonia solani, Corticium rolfsii, and Typhula incarnata^[2].
Tolclofos-methyl (4 μg/mL; up to 26 hours) completely inhibits sporidial multiplication, alters cellular morphology, and causes cytoplasmic loss in Ustilago maydis sporidia after 26 hours, while allowing partial dry weight increase for several hours^[2].
Tolclofos-methyl (1.9-100 μg/mL) inhibits Botrytis cinerea mycelial growth^[2].
Tolclofos-methyl (20 μg/mL; 24 hours) causes cytoplasmic leakage via cell bursting in some Botrytis cinerea germinating conidia^[2].
Tolclofos-methyl (4-8 μg/mL) rapidly inhibits swimming, induces abnormal flagella movement, triggers encystment, and causes cystospore bursting in Phytophthora capsici zoospores^[2].
Tolclofos-methyl (4-8 μg/mL) inhibits swimming, induces circular movement (in Pseudoperonospora cubensis), triggers encystment, and causes cystospore bursting in zoospores of Pseudoperonospora cubensis, Phytophthora infestans, and Plasmopara viticola^[2]. In Vivo:¹⁴C-Tolclofos-methyl (5 mg/kg; p.o.; single dose) results in 87.3-87.7% excretion of radiocarbon within 7 days, less than 1% whole-body residue at 7 days with 3,5-dichloro-4-hydroxybenzoic acid as the main metabolite in Sprague-Dawley rats^[3].
¹⁴C-Tolclofos-methyl (5-50 mg/kg; p.o.; single dose) results in 87.3-90.9% excretion of radiocarbon within 7 days, less than 1% whole-body residue at 7 days with 3,5-dichloro-4-hydroxybenzoic acid and its glycine conjugate as major metabolites in ICR mice^[3].