Crystal Violet (solution)

Crystal Violet (solution) , also known as Gentian violet, methyl violet 10B, is a triphenyl-methane, an alkaline dye that binds to DNA in the nucleus of a cell, staining it a deep purple. It is often used for Gram staining to classify bacteria, or for cell or histological staining^[1].
Solvent and Concentration: Sterile water: 5 mg/mL (0.5%) In Vitro: General Protocol

1.Sample processing
1.1 Paraffin section:
First dewaxing with xylene for 5-10 min, then with fresh xylene, then dewaxing with 100% ethanol for 5 min after dewaxing for 5-10 min. Then 90% ethanol was used for 2 min. At last, 70% ethanol was used for 2 min. DDH₂O 2 min.
1.2 Frozen section:
distilled water for 2 min.
1.3 Tissue culture cells:
fixed with 4% paraformaldehyde for more than 10 min. Wash with distilled water for 2 min, replace with fresh distilled water, and wash again for 2 min.
Note: Other fixatives can be selected according to specific experiments.
2.Crystal violet staining
2.1 After the sample to be tested is treated with the above method, the crystal violet dyeing solution is directly added and dyed for 10 minutes at room temperature (the dyeing solution needs to cover the sample, and the specific dyeing time can be adjusted according to the required dyeing results).
2.2 Wash well in distilled water.
2.3 Dry at room temperature.
2.4 Observe and photograph by light microscope.

Precautions
1. It is recommended to take 1-2 samples for pre-test when using this product for the first time.
2. This product is for R&D use only, not for drug, household, or other uses.
3. For your safety and health, please wear a lab coat and disposable gloves to operate.