(Des-Gly10,D-Ala6,Pro-NHEt9)-LHRH
CAS No. : 52435-06-0
(Synonyms: Surfagon)
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| 5mg | $185 | In-stock |
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| Cat. No. : | HY-P0048 |
| M.Wt: | 1167.32 |
| Formula: | C56H78N16O12 |
| Purity: | >98 % |
| Solubility: | 10 mM in DMSO |
(Des-Gly10,D-Ala6,Pro-NHEt9)-LHRH (Surfagon, Mwt 1167.34 Da) is an agonist of gonadotropin-releasing hormone (GnRH). (Des-Gly10,D-Ala6,Pro-NHEt9)-LHRH can be used as an internal standard for the LC-MS analysis of leuprorelide acetate. (Des-Gly10,D-Ala6,Pro-NHEt9)-LHRH has potential applications in biochemical analysis and fertility[1][2][3].
In Vitro: (Des-Gly10, D-Ala6, Pro-NHEt9)-LHRH as internal standard for leuprorelin acetate[1]
(1) Add 10 μL 200 ng/mL (Des-Gly10,D-Ala6,Pro-NHEt9)-LHRH solution to 100 μL plasma sample.
(2) Add ice-cold acetonitrile to solution in (1) in two batches (500 µL each time).
(3) Stir at 2500 rpm for 10 min.
(4) Centrifugation at 12500 rpm (4℃) for 10min.
(5) Transfer the supernatant into a centrifuge tube, dried under nitrogen gas at 35℃, and finally add 100 µL 0.1% formic acid in water/acetonitrile: 90/10 (v/v) solution.
(6) The Thermo Scientific Vanquish UPLC system is connected to the Thermo Scientific Orbitrap Explorer 480 mass spectrometer. Using the Waters Acquity UPLC HSS T3 column (2.1 × 100 mm, 1.8 µm) separation at a temperature of 40℃.
(7) The mobile phase A is 0.1% formic acid in water, and the mobile phase B is 0.1% formic acid in acetonitrile solution. The gradient starts at 95% mobile phase A, decreases to 10% over 5 min, and then remains constant for 0.5 min. At 6 min, the column returned to 95% mobile phase A and is rebalanced for 2 min. The flow rate was 0.3 mL/min and the injection volume is 20 µL.
(8) The sample is ionized by 3500V electric spray. In positive-ion mode, m/z 605.3275 is leuprolide acetate, and m/z 584.3052 is the internal standard. Data are analyzed by Xcalibur software (version 4.4).
In Vivo: (Des-Gly10,D-Ala6,Pro-NHEt9)-LHRH (2 µg/kg; i.p.; single dose) significantly increases anxiety behavior in adult male rats with and without gonadectomy[2].
(Des-Gly10,D-Ala6,Pro-NHEt9)-LHRH (50 µg; single dose) improves the fertility of cows after fertilization and prevents embryo death when combined with Ainil (30 µL/kg)[3].
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