3-Methyladenine

3-Methyladenine (3-MA) is a PI3K inhibitor. 3-Methyladenine is a widely used inhibitor of autophagy via its inhibitory effect on class III PI3K^[1]. IC50 & Target:IC50: 25 μM (Vps34), 60 μM (PI3Kγ)^[1] In Vitro:3-Methyladenine (0-10 mM; 0-48 hours) induces caspase-dependent cell death in HeLa cells in a time-and dose-dependent manner^[2].
3-Methyladenine (5 mM; 24 hours) suppresses autophagy in HeLa cells under both glucose-free conditions and normal conditions^[2].
3-Methyladenine (5 mM; 0-48 hours) suppresses conversion of LC3-I to LC3-II (autophagy markers) between 12hours and 48 hours, confirms the inhibitory effects on autophagy^[2].
3-Methyladenine induces cell death is independent of autophagy inhibition^[2].
3-Methyladenine significantly shortens the duration of nocodazole-induced-prometaphase arrest^[2].
Note:
The recommended concentration of 3-MA is approximately 0.5-10 mM in cell culture. DMSO stock solution is not recommended. We suggest that you can weigh out the amount of 3-MA you required before your experiment. Then please dissolve it in medium and sterilize with a 0.22 μm filter. In Vivo:3-Methyladenine (1.5 mg/100 g; intraperitoneal injection; 3-24 hours) treatment alleviates sodium taurocholate-induced severe acute pancreatitis (SAP) in rats at both 12 hours and 24 hours^[3].
3-Methyladenine inhibits autophagy of pancreatic acinar cells in sodium taurocholate-tnduced SAP^[3].
3-Methyladenine also shows inhibitory effects on PI3K/Akt signaling pathway and NF-κB signaling pathway in sodium taurocholate-induced SAP^[3].