2(5H)-Furanone

2(5H)-Furanone (γ-Crotonolactone) is an endogenous metabolite. 2(5H)-Furanone mimics N-acyl homoserine lactone signals, occupies the binding site of LuxR homologs, and interferes with quorum sensing-mediated gene regulation. 2(5H)-Furanone inhibits quorum sensing mediated by AHLs with different acyl chain lengths. 2(5H)-Furanone inhibits biofilm formation of environmental Aeromonas hydrophila strains on polystyrene plates. 2(5H)-Furanone suppresses spike-and-wave discharges in a rat model of generalized absence seizures and exhibits selective activity against absence seizures. 2(5H)-Furanone can be used in studies related to bacteria infections and generalized absence seizures.^[1][2] In Vitro:2(5H)-furanone (0-1 mg/mL; 18 h) dose-dependently inhibits short-chain AHL-mediated quorum sensing in Chromobacterium violaceum CV026^[1].
2(5H)-furanone (0.4-1 mg/mL; 12 h) inhibits long-chain AHL-mediated quorum sensing in Agrobacterium tumefaciens NTL-4^[1].
2(5H)-furanone (0.2-1 mg/mL; 6 h) inhibits quorum sensing-mediated biofilm formation of Aeromonas hydrophila on polystyrene surfaces^[1]. In Vivo:2(5H)-Furanone (20-200 mg/kg, i.p.; single dose) dose-dependently inhibits γ-butyrolacton (GBL)-induced generalized absence seizures in adult male Sprague-Dawley rats, with an ED₅₀ of 100 mg/kg. It attenuates the GBL-induced potentiation of generalized absence seizures mediated by Muscimol (HY-N2313) in rats, inhibits GBL-induced generalized absence seizures in male postnatal day 10 to 40 juvenile rats, and suppresses Pentylenetetrazole (PTZ)-induced generalized absence seizures in adult male rats^[2].