Indigo
CAS No. : 482-89-3
| Size | Price | Stock |
|---|---|---|
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| 1g | $40 | In-stock |
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| Cat. No. : | HY-N0335 |
| M.Wt: | 262.26 |
| Formula: | C16H10N2O2 |
| Purity: | >98 % |
| Solubility: | DMSO : < 1 mg/mL (ultrasonic;warming;heat to 60°C);THF : < 1 mg/mL (ultrasonic;warming;heat to 60°C);H2O : < 0.1 mg/mL |
Indigo is a blue dye. Indigo stains cellulose fibers such as cotton. Indigo stains food or biological samples to visualize and quantify the uptake or distribution of substances by organisms[1][2].
In Vitro:Guidelines (The following is our recommended protocol. This protocol is only a guide and should be modified according to your specific needs).
1 Specific staining steps [2]
1) Starve the fruit flies in 0.5% agarose aqueous solution for 18 h to induce hunger.
2) Transfer the hungry fruit flies to standard diet and feed for 15 min to initiate feeding behavior.
3) Transfer the fruit flies to a vial containing Indigo-dyed diet (the Indigo content in the standard diet is 1%) and continue feeding for 15 min.
4) After feeding, observe the color of the fruit fly abdomen under an optical microscope: a blue abdomen indicates that the fruit fly has ingested the indigo-dyed diet, and a white abdomen indicates that the fruit fly has not ingested it.
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