TFB-TBOA

TFB-TBOA (CF3-Bza-TBOA) is a potent glutamate transporter blocker that potently suppresses the activity of glial transporters. TFB-TBOA shows IC₅₀ values of 22, 17, and 300 nM for glutamate transporters EAAT1, EAAT2, and EAAT3 respectively in an uptake assay using cells transiently expressing EAATs^[1]. In Vitro: TFB-TBOA (CF3-Bza-TBOA) inhibits synaptically activated transporter currents (STCs) in astrocytes in the stratum radiatum in rat hippocampal slices in a dose-dependent manner with an IC₅₀ of 13 nM, and reduces them to approximately 10% of the control at 100 nM^[2].
TFB-TBOA inhibits the Na⁺_i response evoked by 200 µM glutamate in a concentration-dependent manner with IC₅₀ value of 43 nM, as measured on the amplitude of the Na⁺_i response^[1].