Bathocuproine

Bathocuproine is a phenanthroline chelator that binds specifically to Cu⁺. Bathocuproine preferentially binds Cu⁺ over Cu²⁺ to form a chromogenic complex, which is used to detect the oxidation state of copper in biomolecules. Bathocuproine undergoes concentration-dependent self-association to form dimers, and it can engage in aromatic stacking interactions with aromatic residues of Aβ (K_d ≈ 1 mM). Bathocuproine is applicable to research related to Alzheimer's disease^[1][2]. In Vitro:Bathocuproine (0.32-3.16 mM) undergoes reversible monomer-dimer self-association in aqueous solution at 25 °C, with an association constant of 0.64 mM^-1.
Bathocuproine (0.32-3.16 mM) binds to the Aβ40 peptide only in its dimeric form in aqueous solution at 25 °C, with a dissociation constant of approximately 1 mM under conditions where the dimer accounts for ≥ 40% of the total^[2].