4-Nitrophenyl phosphate (disodium)
CAS No. : 4264-83-9
(Synonyms: p-Nitrophenyl phosphate (disodium))
| Size | Price | Stock |
|---|---|---|
| 5g | $26 | In-stock |
| 10g | $29 | In-stock |
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| Cat. No. : | HY-15928 |
| M.Wt: | 263.05 |
| Formula: | C6H4NNa2O6P |
| Purity: | >98 % |
| Solubility: | H2O : 160 mg/mL (ultrasonic) |
4-Nitrophenyl phosphate (p-Nitrophenyl phosphate) disodium is a non-proteinaceous chromogenic substrate for alkaline and acid phosphatases used in ELISA and conventional spectrophotometric assays.
In Vitro:1. Equilibrate 4-Nitrophenyl phosphate to room temperature before opening.
2. Dissolve 10 mg of 4-Nitrophenyl phosphate in 10 mL of 1X Diethanolamine (HY-W035903) Substrate Buffer.
Note: Diethanolamine is effective at concentrations from 10 mM to 1 M.
3. Add 100 μL of the 4-Nitrophenyl phosphate solution to each microplate well.
4. Incubate plate at room temperature for 30 minutes, or until sufficient color develops.
5. Add 50 μL of 2 N NaOH to each well to stop the reaction.
6. Measure the absorbance at 405 nm. Alternatively, monitor reaction in a kinetic ELISA reader.
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Inquiry Information- Product Name:
- 4-Nitrophenyl phosphate (disodium)
- Cat. No.:
- HY-15928
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