14-Deoxyandrographolide (Standard)

14-Deoxyandrographolide (Standard) is the analytical standard of 14-Deoxyandrographolide. 14-Deoxyandrographolide is a diterpene with calcium channel blocking activity and acts as a uterine smooth muscle relaxant. 14-Deoxyandrographolide stimulates the release of nitric oxide (NO) in endothelial cells. 14-Deoxyandrographolide gradually desensitizes liver cells to TNF-α mediated apoptosis by inducing the release of TNFRSF1A^[1][2][3]. In Vitro:14-Deoxyandrographolide can reduce uterine smooth muscle contraction responses induced by 0.3 mM and 3.0 mM CaCl², with IC₅₀ values of 1.24 M and 5.94 M^[1].
14-Deoxyandrographolide (0-15 nM, 1 h) shows no cytotoxicity in primary liver cells and prevents TNF-α induced cell death^[2].
14-Deoxyandrographolide (5-15 nM, 1 h) inhibits the expression of caspase-3 in primary liver cells^[2].
14-Deoxyandrographolide (10 nM, 1 h) suppressed the aggregation of TRADD, FADD and caspase-8 in TNF-a sensitized liver cells and inhibited the formation of the DISC^[2].
14-Deoxyandrographolide (10 nM, 1 h) induces the release of TNFRSF1A from liver cells^[2].
14-Deoxyandrographolide (0-100 μM, 24-48 h) stimulates endothelial cells to produce NO in a concentration-dependent manner^[3]. In Vivo:14-Deoxyandrographolide (40 mg/kg, intraperitoneal injection, single dose) makes rat liver cells resistant to apoptosis induced by TNF-α by downregulating the formation of death-inducing signaling complex. It enhances the activity of microsomal Ca-ATPase and increases calcium ion influx into the microsomal lumen through the NO/cGMP pathway, which in turn promotes the release of TNFRSF1A^[2].