Timosaponin AIII

Timosaponin AIII could inhibit acetylcholinesterase (AChE) activity, with an IC₅₀ of 35.4 μM. IC50 & Target:IC50: 35.4 μM (AChE)^[1]. In Vitro: Timosaponin AIII could inhibit acetylcholinesterase (AChE) activity, with an IC₅₀ of 35.4 μM^[1]. Timosaponin AIII is identified as a major selective cytotoxic activity in BN108, and its selective cytotoxic activity involves inhibition of mTOR, induction of ER stress and protective autophagy^[2]. In Vivo: Of the tested steroidal saponins, Timosaponin AIII (TA3) most potently improves memory deficits. Timosaponin AIII increases the scopolamine-induced reduction in step-through latency by 17% (10 mg/kg), 28% (20 mg/kg), and 43% (40 mg/kg). During the acquisition trial, no differences in latent time are observed. Timosaponin AIII (20, 40 mg/kg, p.o.) potently inhibits this reduction of acetylcholine in scopolamine-treated mouse brain. The inhibitory effect of Timosaponin AIII is comparable to that of tacrine, which is used as a positive control^[1].