BMPO

BMPO (BocMPO) is a cell-permeable superior spin trap with favorable chemical and spectroscopic features. BMPO (BocMPO) can be used for detecting thiyl radicals, hydroxyl radicals, superoxide anions and glutathiyl radicals^[1]. In Vitro:BMPO is able to trap both ?OH and GS? to form more persistent BMPO/?OH and BMPO/?SG adducts. In addition, the electron spin resonance (ESR) spectra of BMPO-derived adducts exhibit high signal-to-noise ratios in biological systems. For example, the ESR spectrum of the BMPO glutathionyl adduct (BMPO/?SG) does not fully overlap with the spectrum of its hydroxyl adduct. The favorable chemical and spectroscopic features make BMPO ideal for the detection of superoxide anions, hydroxyl and thiyl radicals.^[1]
The potential toxic effects of the spin trap BMPO is measured by two estimates of cell viability (trypan blue exclusion and colony formation).^[3]
BMPO has no significant effect on cell viability at 2.5 or 25 mM in CHO cells. However, it leads to a significant increase in the number of cells that are unable to exclude Trypan blue at 50 mM BMPO. BMPO significantly reduces a 30% reduction in colony formation at 25 mM concentration in CHO cells. And, BMPO completely inhibits the colony formation of 9L tumor cells at 50 mM.^[3]
The cell function (rate of oxygen consumption) is also measured, BMPO (25 mM) significantly reduces the oxygen consumption rates in CHO cells.^[3]