Fluorometholone acetate

Fluorometholone acetate is a synthetic glucocorticoid corticosteroid and a corticosteroid ester. Fluorometholone acetate potently inhibits carbonic anhydrase (CA) with IC₅₀s of 2.18 μM and 17.5 μM for hCA-I and hCA-II, respectively. Fluorometholone acetate has anti-inflammatory effect and has the potential for external ocular inflammation research^[1][2][3]. IC50 & Target: IC50: 2.18 μM (human carbonic anhydrase (hCA)-I) and 17.5 μM (hCA-II)^[2]
Ki: 1.044 μM (hCA-I) and 9.98 μM (hCA-II)^[2] In Vitro: For Fluorometholone acetate, a K_i value from Lineweaver-Burk plots is obtained as 1.044 μM (noncompetitive) for hCA-I and 9.98 μM (non-competitive) for hCA-II^[2]. In Vivo: Hourly topical administration of 0.1% Fluorometholone acetate ophthalmic suspension produced, on the average, a 47% reduction in the polymorphonuclear leukocytes invading the cornea during an experimentally induced inflammatory keratitis in New Zealand albino rabbits (1.5-2.0 kg). Fluorometholone acetate (0.1%) formulated as a high-viscosity carbomer gel and applied at three-hour intervals reduced invading leukocytes in the cornea an average of 48%, an effect not significantly different from hourly administration of the suspension^[1].