2,4,6-Tri-2-pyridinyl-1,3,5-triazine

2,4,6-Tri-2-pyridinyl-1,3,5-triazine (NSC 112125) is a colorimetric reagent for detecting iron. 2,4,6-Tri-2-pyridinyl-1,3,5-triazine forms a complex with Fe (II) and can be quantified as a measure of iron concentration by colorimetric detection at 594 nm^[1]. In Vitro:The intense violet color formed by 2,4,6-Tri-2-pyridinyl-1,3,5-triazine (TPTZ) with Fe (II), with only two of the three pyridyl groups of each 2,4,6-Tri-2-pyridinyl-1,3,5-triazine ligand attached to the iron atom^[1].

Guide (The following is our recommended protocol. This protocol is only a guide and should be modified according to your specific needs).
Standard ironsolutions are prepared by dissolving electrolytic iron in hydrochloric acid and diluting to the desired volume.
A 10% solution is prepared by dissolving 100 g of Hydroxylammonium chloride in 900 mL of water.
A solution 2 M in Sodium acetate and 2 M in Acetic acid (HY-Y0319) is prepared by dissolving 164 g of Sodium acetate and 115 mL of Acetic acid in water, and diluting to 1 L.
A series of solutions containing excess 2,4,6-Tri-2-pyridinyl-1,3,5-triazine and an iron concentration varying from 0 to 6 X 10^-6 M is prepared by pipetting aliquots of a 1.000 X 10^-4 M iron solution into 50 mL volumetric flasks and adding 5.0 to 10.0 mL of 0.001 M 2,4,6-Tri-2-pyridinyl-1,3,5-triazine to each solution.
The molar ratio of 2,4,6-Tri-2-pyridinyl-1,3,5-triazine to iron is greater than three in each solution.
2 mL of 10% hydroxylammonium chloride is added to reduce any iron (III) and each solution is buffered with 10 mL of Sodium aretate-Acetic acid solution.
The absorbancy of each solution is determined at 593 nm using 1 cm cells.