3-Indoxyl phosphate (sodium)
CAS No. : 3318-43-2
(Synonyms: 3-IP (sodium))
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| Cat. No. : | HY-W009458 |
| M.Wt: | 257.09 |
| Formula: | C8H6NNa2O4P |
| Purity: | >98 % |
| Solubility: |
3-Indoxyl phosphate (3-IP) is a chromogenic and electrochemical substrate for alkaline phosphatase. Upon enzymatic cleavage by alkaline phosphatase, 3-indoxyl is released and subsequently oxidized to produce water-insoluble indigo, which can be quantified by colorimetric detection at 660 nm as a measure of alkaline phosphatase activity. 3-Indoxyl phosphate can also be converted to water-soluble leucoindigo by reduction of indigo in alkaline solution by sodium dithionite, which can be quantified by colorimetric detection at 415 nm or voltametric detection as a measure of alkaline phosphatase activity.2 3-Indoxyl phosphate has been used in ELISAs.
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