Dansyl hydrazine

Dansyl hydrazine is a carbohydrate-specific fluorescent dye (Ex/Em = 340 nm/525 nm). Dansyl hydrazine undergoes a condensation reaction with aldehyde groups generated by periodate oxidation on carbohydrate-containing structures to form fluorescent hydrazone compounds. Dansyl hydrazine selectively stains polysaccharides in formalin-fixed, paraffin-embedded human post-mortem brain tissues, revealing detailed structural features. Dansyl hydrazine is applicable to research related to Alzheimer's disease, Lafora disease, and polyglucosan body disease^[1][2][3]. In Vitro:Dansyl hydrazine (5 mg) enables sensitive, low-background fluorescent staining of polyglucosan bodies (amyloid bodies in Alzheimer's disease/aged brains, Lafora bodies in Lafora disease, and polyglucosan bodies in adult polyglucosan body disease) in formalin-fixed, paraffin-embedded human brain tissues, and reveals unique structural details of each body type^[1].
Dansyl hydrazine (1%) forms highly fluorescent hydrazones with aldoses (including D-glucose), and the products can be quantitatively analyzed by thin-layer chromatography (TLC) and fluorometry^[2].
Dansyl hydrazine serves as the starting material for Dz-Jul, a Zn²⁺ -selective fluorescent chemosensor. It enables the detection of Zn²⁺ in solutions and water samples, and also allows qualitative monitoring via test strips^[3].

Guide (The following is our recommended protocol, which serves only as a guide and should be modified according to your specific needs).
Fluorescent Staining of Polysaccharides in Tissue Sections^[1]
1. Applicable Scenarios: Specific labeling of corpora amylacea, Lafora bodies, glycogen, etc., in paraffin sections.
2. Reagent Preparation:
Periodic acid solution: 0.7% periodic acid (dissolved in 5% aqueous acetic acid).
Dansyl Hydrazine working solution: Dissolve 5 mg dansyl hydrazine in 1.67 mL absolute ethanol, then add 3.33 mL 0.1 M sodium acetate buffer (pH 5.5) and mix well (final concentration is approximately 1 mg/mL).
3. Procedure:
Deparaffinization and hydration: Deparaffinize paraffin sections with xylene, then rehydrate through a graded ethanol series (100%-95%-80%-70%) to water.
Oxidation: Immerse sections in 0.7% periodic acid solution and incubate at room temperature in the dark for 1 hour.
Washing: Rinse with distilled water 3 times, 10 minutes each time.
Labeling: Add Dansyl Hydrazine working solution to cover the tissue, and incubate at room temperature in the dark for 1 hour.
Washing: Rinse with distilled water 3 times, 10 minutes each time.
Mounting: Dehydrate through a graded ethanol series (70%-80%-95%-100%), replace the dehydrant in the tissue with an organic solvent such as xylene to render the tissue transparent, then mount the sections with a fluorescent mounting medium such as Acrymount or DPX.
4. Result Observation:
Excitation/emission: Use a BV-2A or similar filter.
Fluorescence color: Polysaccharide structures show bright yellow-green fluorescence against a black background.