Clomipramine

Clomipramine (Chlorimipramine) is a potent 5-HT reuptake blocker with the IC₅₀ value of 1.5 nM. Clomipramine is a tricyclic antidepressant that can be used for the research of depression and obsessive compulsive disorder (OCD)^[1]. In Vitro: Clomipramine can inhibit reuptake of both noradrenaline and 5-HT, although Clomipramine inhibits 5-HT reuptake more strongly than it inhibits noradrenaline reuptake^[1].
The antidepressant Clomipramine inhibits both venom AChE as well as human serum BChE in a concentration-dependent manner but has no effect on AChE in the rat brain striatum^[2]. Clomipramine interferes with the autophagic flux and severely compromises the viability of tumorigenic cells upon cytotoxic stress^[3].
Clomipramine reduces autophagy in neuronal primary cultures. Clomipramine (1 and 5?μM) negatively regulates neuronal autophagic pathway in primary cultured cells^[3]. In Vivo: Clomipramine (5-20 mg/kg; i.p) elicits significant hyperglycemia in mice. Clomipramine induces hyperglycemia in mice by blocking the 5-HT2B and/or 5-HT2C receptors, which results in facilitation of adrenaline release. In mice, Clomipramine reduces immobility in the forced swimming test, which is the behavioral model for antidepressants. Clomipramine also inhibits the OCD animal model, marble burying behavior in mice^[1]. Clomipramine (20?mg/kg) decreases autophagic flux in murine tissues^[3].