Robinin

Robinin is a flavonoid that can be extracted from the leaves of purple cowpea, inhibiting TGF-β, TLR4/NF-κB and TLR2-PI3k-AKT signaling pathways. Robinin exerts anti-inflammatory and anti-tumor effects. The combination of Robinin and Methotrexate (HY-14519) reduces inflammation in experimental arthritis, Robinin can decrease the Doxorubicin (HY-15142A) induced cardiac toxicity effect^[1][2][3][4]. In Vitro:Robinin (1-10 μg/ml, 24 h) shows no obvious cytotoxicity in hPBMCs cells^[1].
Robinin (6 μg/mL, 24 h) inhibits the expression of MCP1, TNF-α, IL-6 and ICAM-1 proteins and the mRNA levels of TLR2 and TLR4 in hPBMCs cells, and had anti-inflammatory effects ^[1].
Robinin (1 μM, 24 h) inhibits the proliferation and migration of Mia-PACA2 and PANC-1 cells^[2].
In Vivo:The combination of Robinin and methotrexate (50 mg/kg and 0.3 mg/kg, Oral gavage, Twice a week) has anti-inflammatory and anti-arthritic action in adjuvant arthritis mice models^[3].
Robinin (50 mg/kg, Oral gavage, 10 days) can modulate the cardioprotective effect of the TGF-β1 signaling pathway on Adriamycin-induced cardiotoxicity^[4].