Negletein

Negletein (5,6-Dihydroxy-7-methoxyflavone) is a flavone found in Scutellaria. Negletein shows anti-inflammatory activity via inhibiting TNF-α and IL-1β with IC₅₀ values of 16.4 and 6.4 μM, respectively. Negletein can activate Nrf2 and inhibit ROS production. Negletein can enhance the neuroprotective effect of nerve growth factor. Negletein can inhibit amyloid beta-peptide release and accumulation. Negletein can inhibit pathogens biofilms formation. Negletein can be used for the researches of cancer, infection, inflammation and neurological disease, such as colon cancer and Alzheimer's disease^{[1][2][3][4][5]}. In Vitro:Negletein (10 nM-10 mM) does not induce neurite outgrowth in PC12 cells^[1].
Negletein (10 μM, 3 days) trigger PC12 cells to differentiate and extend neuritis companied with nerve growth factor (5 ng/mL)^[1].
Negletein (10 μM) increases p-ERK, p-Akt, p-CREB, and GAP-43 levels companied with nerve growth factor (5 ng/mL) in PC12 cells^[1].
Negletein (10 nM-10 μM, 24-72 h) increases survival rate (cell viability) of PC12 cells cultured in serum-free medium^[1].
Negletein (20 μM) inhibits APP expression, β-Secretase activity and Aβ42 release in iron-induced SHSY5Y cells^[3].
Negletein (5-20 μM) inhibits hydroxyl radical formation in iron-induced SHSY5Y cells^[3].
Negletein (20 μM, 24 h) prevents oligomerization of amyloid Aβ42 in iron-induced SHSY5Y cells^[3].
Negletein (5-50 μM, 4 h) increases Nrf2 expression in HCT116 cells^[4].
Negletein (0-30 μM, 4 h) reduces ROS production in HCT116 cells^[4].
Negletein shows MIC of 32 μg/mL against S. aureus and Bacillus sp.^[5].